A Comparative Analysis of CRISPR-Cas Systems and Traditional Gene Editing Techniques in Eukaryotic Cells
Keywords:
CRISPR-Cas systems, gene editing, eukaryotic cells, Zinc Finger Nucleases, Transcription Activator-Like Effector Nucleases, genetic engineering, biotechnology, off-target effectsAbstract
Gene editing has revolutionized molecular biology, yet the choice between CRISPR-Cas systems and traditional methods such as ZFNs and TALENs remains contentious. This study employs an experimental design to evaluate the efficiency, specificity, and off-target effects of these approaches across various eukaryotic models, including murine and plant systems. Utilizing quantitative measurements of editing accuracy through next-generation sequencing and comparative analysis metrics, we demonstrate that CRISPR-Cas systems significantly outperform traditional methods in terms of efficiency (up to 90% vs. 60%) and reduced off-target effects (p < 0.01). Our findings elucidate the advantages and limitations of each technique, offering critical insights for researchers in the field of genetic engineering.
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