A Comparative Analysis of CRISPR-Cas9 and RNA Interference Mechanisms in Gene Expression Modulation

Taylor Williams; Olivia Morris; Nico Wilson; Ashley Taylor

Authors

Taylor Williams PhD
Olivia Morris Professor
Nico Wilson Associate Professor
Ashley Taylor Dr. Sc

Keywords:

CRISPR-Cas9, RNA interference, gene expression modulation, genome editing, therapeutic intervention, genetic disorders, off-target effects, functional genomics

Abstract

This study investigates the differential efficacy of CRISPR-Cas9 and RNA interference (RNAi) in modulating gene expression, addressing their applications in therapeutic settings. Utilizing a structured comparative framework, this analysis evaluates target accessibility, off-target effects, and phenotypic outcomes. Results highlight CRISPR-Cas9's superior precision, while RNAi demonstrates robust silencing efficacy in specific contexts. The findings enhance our understanding of genetic tool selection, offering insights into optimizing therapeutic interventions for genetic disorders.

Author Biographies

Taylor Williams, PhD

PhD
Massachusetts Institute of Technology
77 Massachusetts Avenue, Cambridge, MA 02139, USA

Olivia Morris, Professor

Professor
University of Cambridge
The Old Schools, Trinity Ln, Cambridge CB2 1TN, UK

Nico Wilson, Associate Professor

Associate Professor
University of Sydney
Camperdown NSW 2006, Australia

Ashley Taylor, Dr. Sc

Dr. Sc
Ludwig Maximilian University of Munich
Geschwister-Scholl-Platz 1, 80539 München, Germany

References

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