Harnessing CRISPR-Cas9 Synergy with Metabolic Engineering to Mitigate Antibiotic Resistance in Pseudomonas aeruginosa
Keywords:
CRISPR-Cas9, metabolic engineering, antibiotic resistance, Pseudomonas aeruginosa, genome editing, efflux pumps, metabolic pathways, antimicrobial therapyAbstract
The emergence of multidrug-resistant Pseudomonas aeruginosa strains poses a significant threat to global public health. By employing CRISPR-Cas9 technology coupled with metabolic engineering, this study explores a novel approach to suppress resistance mechanisms. We engineered strains with targeted disruptions in critical resistance-conferring genes, leading to enhanced antibiotic susceptibility. The findings demonstrate the potential of integrating gene-editing and metabolic pathways to address antibiotic resistance effectively. This research underscores a promising strategy for future antimicrobial development.
References
Yusif, S. H., Shukurova, Z. Y., Ismailova, A. C., & Azizov, F. S. (2016). Ultraviolet and visible spectroscopy for the analysis of crude extract of Cotinus coggygria (Cotinus coggygria Scop; Anacardiaceae).
Kumar, R., & Shukurova, Z. Y. (2018). Wild silk moths’ conservation status in India. Proc of the GRI of ANAS, 7(1), 122-129.
Shukurova, Z. Y., & Shukurlu, Y. H. (2024). Retraction Note: Non-mulberry silkworm Saturnia Pyri (Denis & Schiffermüller, 1775) and a new perspective source of biomaterials.
